TankblotFor gentle blotting of large and temperature-sensitive proteins
- Simultaneous blotting of up to four 10 x 10 cm gels
- Integrated cooling-jacket for effective cooling
- Homogeneous buffer temperature
Electro-blotting is an important electrophoresis method to transfer mainly proteins from polyacrylamide gels on to nitrocellulose or other carrier membranes. During tank blotting proteins are transferred in a vertical buffer tank between coiled platinum wire electrodes arranged on two sides. For this technique, the gel and blotting membrane are clamped in a frame between filter paper and fiber pads.
In the Tankblot a cooling jacket is integrated into the sidewalls. This results in convective chilling by circulation: the temperature is distributed more homogeneously than with other designs (? 0.5?C at 6?C buffer temperature). This special design also prevents formation of ion gradients.
The Tankblot is recommended particularly for blotting of large molecules (> 100 resp. 200 kDa, depending on the proteins' composition) or of proteins which are difficult to transfer. The Tankblot is the instrument of choice also for native (temperature-sensitive) enzymes.
By selecting an appropriate buffer, transfer can be fast (between 30 min. and 4 h) or, under exceptionally gentle conditions, overnight (ca. 12 h). Thanks to the buffer volume of 1.2 liters transfer times of more than 24 h can be realised, e. g. for gentle blotting of very big proteins.
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Core compentencies
Electrophoresis
